BNS is an efficient thermophotosensitive genic male sterile line of wheat (Triticum aestivum). It is sterile in low temperature and fertile in high temperature. With these features, BNS was widely used in hybrid wheat breeding. In this study, based on the gene chip data of the anthers of BNS sterile line and fertile line, we found that the transcription factor TaERF7 was differentially expressed between the two lines, and cloned the cDNA and promoter sequences of TaERF7，analyzed TaERF7 response to temperature and day length by qRTPCR. The results showed that: (1) bioinformatics analysis revealed that the coding sequence (CDS) of TaERF7 is 660 bp and encoding of 219 amino acids. TaERF7 protein contains AP2 domain and two EAR motifs. It belongs to class Ⅱ ethylene response factors (ERFs). The amino acid sequence of TaERF7 is homologous to AtERF4 in Arabidopsis (Arabidopsis thaliana), which is a transcriptional repressor. The promoter region contains multiple cisacting elements with light and low temperature response. (2) The qRTPCR results showed that TaERF7 expressed in different tissues of BNS. The expression of TaERF7 was downregulated 0.47 fold under long daylight (14 h), while it was upregulated 1.14 fold under short day (10 h). The low temperature treatment at 4 ℃ increased the expression of TaERF7 about 25.7 fold in 2 h, and maintained a high level upto 48 h. The high temperature treatment at 37 ℃ increased the expression of TaERF7 about 0.71 fold in 1 h, but it was sharply downregulated to 0.85 fold after 2 h. According to the above results, we speculated that TaERF7 combined to cisacting element of downstream genes, regulated their expression, and caused the male sterility in BNS.