QTL Mapping of Drought and Salt Tolerant Traits in Wheat at Germination Stage
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    Abstract:

    In this study, the test material was carried out using RILs (recombinant inbred lines) derived from the cross of ‘Shannong 0431 × Lumai 21’. Drought and salt environments were simulated with 20% PEG6000 solution and 100 mmol·L-1 NaCl, respectively. We analyzed quantitative trait loci (QTL) for drought and salt tolerant related traits in wheat on the 12 drought and salt tolerant traits in wheat germination, combining with a genetic map constructed by our group, to provide references for the cloning of salttolerant and droughtresistant genes and markerassisted selection of wheat. The results showed that: (1) a total of 143 QTLs were detected for 12 traits under normal, drought and salt environments. A number of 29 RHFQTLs were detected with the average contribution rate ranged from 4.39% to 13.28%. Among them, the contribution rate of 10 RHFQTLs was more than 10%. (2) A total of 17 and 8 RHFQTLs with specific expression were detected under stress and normal treatments, respectively. Four RHFQTLs with stable expression were detected. (3) A number of three QTL clusters formed by 7 RHFQTLs were detected, distributing on chromosomes 2D, 4D and 5B. Among them, QC1 was located in the wPt6847-D1172783 of 2D chromosome, including three QTLs (QRl2D.2, QSdw2D.3, QTdw2D). QC2 was located in the D2245724-D1108531 of 4D chromosome, including two QTLS (QSl4D, QShl4D). QC3 is located in D982263-S1083095 of chromosome 5B chromosome, including two QTLS (QSl5B.2, QTdw5B.1).

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WANG Xiuhua, YU Hongxiang, SUN Fulai, PAN Xiangyu, ZHAO Yan, LI Sishen. QTL Mapping of Drought and Salt Tolerant Traits in Wheat at Germination Stage[J]. Acta Botanica Boreali-Occidentalia Sinica,2020,40(2):240-251

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  • Online: May 02,2020
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