With the fluorescent lamp (16 mm diameter, 28W, light intensity 9.45 μmol·m^-2·s^-1) as light source to set up four supplementary treatments, including dark (T₀，without lamp) and low (T₁，one lamp), medium (T₂，two lamp) and high (T₃，three lamp) light intensities (8 h·d^-1), we simulated a onemonth of storage of Bougainvillea spectabilis ‘Crimsonlake’, and measured the defoliation rate, photosynthetic pigment content, cell membrane permeability, antioxidant enzyme activity and other related physiological and biochemical indexes of leaves, so as to study the effect of supplementary treatments on the leaves of B. spectabilis and provide the basis for solving the serious defoliation problem during storage and transportation of B. spectabilis. The results showed that: (1) on the 28th day of simulated storage, the defoliation rates of T₀ and T₃ treatments were serious, reaching over 87%, and the defoliation rates of T₁ and T₂ treatments were significantly lower than that of T₀ and T₃ treatments, only 16.33% and 16.92%; The specific leaf weight of T₁~T₃ treatments is higher than that of T₀ treatments, and the relative water content is lower than that of T₀ treatments. (2) The relative conductivity, MDA content, soluble protein content of T₁ and T₂ treatments were significantly lower than that of T₀ treatment. (3) The contents of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid in leaves of T₀, T₂ and T₃ treatments increased firstly and then decreased, while that of T₁ treatment showed an upward trend with small changes. (4) During the whole storage and transportation period, the activity of superoxide dismutase (SOD) in the leaves showed a gradual increase, while the activities of catalase (CAT) and peroxidase (POD) increased firstly and then decreased. There was no significant difference in the CAT activity of leaves between T₀, T₁ and T₂ treatments. POD activity of leaves treated with T₁ and T₂ was significantly higher than that of T₀ treatment. On the 28th day, the SOD activity in the leaves of T₂ treatment was significantly higher than that of T₁ treatment, which increased by 60.86% and 45.02%, respectively, compared with the 0th day. The research indicated that the light intensity of 9.45 μmol·m^-2·s^-1, i.e. a fluorescent lamp, could be used to compensate light for 8 h·d^-1 on the B. spectabilis during storage, which could reduce damages of the cell membrane and influences of the photosynthetic pigment caused by the dark stress in the process of storage. It was beneficial to reduce the wastage of photosynthetic product in the leaves and the yellowing of the leaves, so that the B. spectabilis could grow normally during storage to reduce economic losses.