By comparing the differences in tissue anatomy, membrane permeability, and endogenous hormones of different varieties of jujube kiwifruit during the natural wintering period, we investigated the adaptability of kiwifruit to low temperature in order to provide a theoretical reference for the selection and identification of coldresistant kiwifruit varieties.The fiveyearold kiwifruit cultivars ‘Kuilv’, ‘Fenglv’ and ‘966’ were used to determine the changes in anatomical structure of shoot tissue, cell membrane permeability and endogenous hormones such as GA₃, ABA, and IAA during the winter. We hope to determine the lowtemperature adaptability and its underlying mechanism of different varieties of kiwifruit. The result shows: (1) the xylem ratio from big to small is: ‘Kuilv’, ‘966’and ‘Fenglv’, and the cortex ratio is ‘Fenglv’, ‘966’and ‘Kuilv’. The anatomy of ‘Kuilv’ is significantly different from the other two varieties. (2) The relative electrical conductivity and MDA content of kiwifruit showed a trend that firstly increased and then decreased when temperature changes, and reached the highest in January that the temperature was the lowest. Among them, ‘Kuilv’ has the smallest change, and ‘Fenglv’ is affected by low temperature. (3) As the temperature dropped, GA₃ and IAA content gradually decreased, and ABA content gradually increased from November to January. With the change of natural temperature from November to February, the contents of GA₃ and IAA both decreased firstly and then increased, reaching the lowest value in January, and the ABA content increased firstly and then decreased, reaching a peak in January. Low temperature has a significant impact on the tissue anatomy, membrane permeability and endogenous hormones of three A. arguta varieties. Variety ‘Kuilv’ has the largest ratio of xylem and smallest ratio of cortex, and the smallest change in relative conductivity and MDA content.The endogenous hormone content GA₃ and ABA/GA₃ have a smaller change trend, so their cold resistance is stronger than that of ‘Fenglv’ and ‘966’.