Perennial ryegrass (Lolium perenne) is a coldseason gramineous species, and its distribution and yield are severely affected by temperature stress. Transcription factors (TFs) can regulate gene expression, playing important roles in plant acclimation to stress. Here, we used the perennial ryegrass cv Yatsyn to analyze the TF genes and their response patterns under heat (40 ℃), cold (-10 ℃), control (22 ℃) by highthroughput RNA sequencing, and to screen adverse tolerance candidates of TF genes. The results showed (1) 694 TFs unigenes were identified, belonging to 32 families such as AP2/ERF, GTF, HSF, MYB, NAC, WRKY, bHLH and bZIP, etc. (2) Upon heat and cold stresses, the gene expressions of most members of the ERF (AP2/ERF), MYB, NAC and bZIP families increased, while the WRKY family decreased. In addition, most members of the HSF, GTF and DREB (AP2/ERF) family genes were upregulated by heat, while most bHLH family members elevated by cold. (3) The functional enrichment results indicated that temperatureresponsive TFs were mainly involved in plant hormone signaling, plant circadian rhythms, and plantpathogen interaction. In general, the TF genes involved in stress adaptation were usually upregulated, while most genes involved in growth and disease resistance were downregulated. This study revealed a corner of the response mechanism of plants to temperature stress at the transcriptional level, and provided substantial candidate tolerance genes for the improvement of perennial ryegrass and other plants.