In order to explore the function of wheat (Triticum aestivum L.) WRKY gene, we cloned two WRKY genes named TaWRKYⅢA37 and TaWRKYⅡcD2 from wheat variety ‘Kenong 199’ by homologous cloning, and their bioinformatics and expression under different stresses were analyzed. Bioinformatic analysis showed that TaWRKYⅢA37 and TaWRKYⅡcD2 genes contain two introns and three exons, encoding 206 and 138 amino acids, respectively. The encoded proteins are all hydrophilic unstable non secretory nucleoproteins. Phylogenetic analysis showed that TaWRKYⅢA37 protein was the closest to its two homologous copies, while TaWRKYⅡcD2 protein was the closest to that of Aegilops tauschii. The results of qRTPCR showed that TaWRKYⅢA37 and TaWRKYⅡcD2 genes were expressed in the roots, stems and leaves of wheat. The former had the highest expression level in the roots, while the latter had the highest expression level in the leaves, all of which were low expression in the stems. The expression of TaWRKYⅢ_A37 gene in seedling stage was upregulated by PEG, H₂O₂ and ABA stress, and down regulated within 4~8 hours after NaCl treatment, which was lower than that of the control; While the expression was up regulated by PEG, NaCl, H₂O₂ and ABA in the filling stage. At seedling stage, TaWRKYⅡcD2 gene was down regulated by NaCl, H₂O₂ and ABA stress, and up regulated by PEG treatment for 2 hours, which was higher than that of control; While the expression was down regulated by PEG and NaCl stress at the filling stage, and up regulated by H₂O₂ and ABA stress. The results of this study lay a theoretical foundation for further exploring the antistress function of TaWRKYⅢA37 and TaWRKYⅡcD2 genes.