Metal tolerant protein (MTP) maintains metal homeostasis in plants by absorbing and expelling metals from the cytoplasmic sol. In this study, we used a variety of bioinformatic methods to identify and analyze the TaMTP genes in the wheat genome, and qRTPCR to analyze the expression of TaMTP genes under the stress of various heavy metals, so as to provide theoretical basis for indepth study of the regulatory mechanism and stress resistance of this family of genes on the growth and development of wheat. The results showed that: (1) TaMTPs all had cationic Efferent family domain, and most members had zinc transporter dimeric domain. Phylogenetic and cluster analysis showed that TaMTP proteins were divided into seven groups: G1, G5, G6, G7, G8, G9 and G12. Analysis of gene structure and motif revealed that most TaMTP genes had relatively conservative exonintron arrangement and conserved motif composition. (2) Gene expression profile analysis of RNASEQ data showed that different TaMTP genes had their own unique expression mechanism, among which TaMTP11Bb and TaMTP11D had higher expression levels under abiotic stress, TaMTP11A, TaMTP11Bb, TaMTP11D, TaMTP113Ab, TaMTP113B and TaMTP113D had higher expression levels under biological stress. (3) qRTPCR analysis showed that when wheat was subjected to heavy metal stress of zinc (Zn), copper (Cu), cobalt (Co), cadmium (Cd), manganese (Mn) and iron (Fe), the expression levels of TaMTP11A、TaMTP84A、TaMTP84D、TaMTP113Aa and TaMTP113B were increased. The results showed that each metal ion could induce the expression of these TaMTP genes in roots and leaves. However, the expressions of TaMTP11A and TaMTP84A under Fe³⁺ and Cu²⁺ stress were completely opposite, and the expression levels of the two genes in wheat leaves and root tissues were low under Fe³⁺ stress. It was speculated that TaMTPs might be involved in the tolerance or transport of corresponding trace elements, but different TaMTP had different transport functions for different metals.