Identification and Expression Analysis of Alfinlike Transcription Factor Family in Grape
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    Abstract:

    The Alfinlike (AL) transcription factor family plays an important role in abiotic stress such as high salt, low temperature, and drought. In this study, the homology ratio method was used to retrieve and identify the genes of grape AL transcription factor family, analyze their bioinformatics characteristics and the expression characteristics of AL gene under abiotic stress by qRTPCR, so as to explore the function of grape AL gene under abiotic stress. The results showed that: (1) we identified 6 members of the AL gene family in grape, they were named VvAL1-VvAL6, and the 6 members were distributed on 6 chromosomes. (2) The AL transcription factors in grape had highly conserved DUF3594 domain and PHD domain and each gene contained 5 exons and 4 introns. The upstream promoter region analysis showed that there was a lot of ciselements associated with various stress and hormone responses. (3) Gene chip expression pattern analysis showed that salt, drought, ABA stress and low temperature (5 ℃) treatments all significantly affected the expression of AL family genes (VvAL1-VvAL6). (4) qRTPCR showed that the expression level of AL gene in grape leaves was different under different stress treatments; under ABA treatment, the gene expression of grape AL transcription factor family was significantly downregulated compared with CK, while PEG treatment not significant. Under salt stress, the expression levels of VvAL2, VvAL4 and VvAL5 genes were significantly upregulated, which were 23 times, 8.5 times and 10.5 times of the control, respectively. The expression levels of VvAL1 and VvAL6 genes were significantly downregulated, which were 33 times and 25 times of the control, respectively. It was found that grape AL transcription factor family is closely related to plant hormone and abiotic stress, especially the genes of this gene family strongly respond to high salt stress.

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XIE Min, WANG Ping, HE Honghong, CAO Xuejing, MAO Juan, CHEN Baihong. Identification and Expression Analysis of Alfinlike Transcription Factor Family in Grape[J]. Acta Botanica Boreali-Occidentalia Sinica,2020,40(9):1467-1474

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  • Online: October 10,2020
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