In order to explore the function of wheat WRKY gene, we cloned a WRKY gene from wheat leaves using RTPCR, and analyzed its bioinformatics and expression under different stresses . The results showed that： (1) the WRKY gene of wheat was successfully cloned, and was designated as TaWRKY47. (2) Sequence analysis showed that the length of TaWRKY47 open reading frame (ORF) was 900 bp, encoding 299 amino acids. The amino acid sequence contained a conserved WRKY domain and a zinc finger structure (C₂HC), belonging to Group of WR8aKY Ⅲ family. (3) The subcellular localization analysis showed that TaWRKY47 was located in cell nucleus. (4) Expression pattern showed that TaWRKY47 gene was expressed in roots, stems, leaves, pistils and stamens, with the highest expression found in pistils. The expression of TaWRKY47 cloud be strongly induced by low temperature, drought, salt, ABA and H₂O₂ stresses. It is suggested that TaWRKY47 gene may play a positive role in stress resistance of wheat. The results provided a theoretical foundation for further study of TaWRKY47 gene function and the antistress mechanism.