WRKY transcription factor is one of the most important transcription factors for plants in response to pathogenic bacteria stress, and participates in disease resistance and the regulation of signal transduction pathways. In order to study the biological characteristics of pepper WRKY genes, we used the pepper highly resistant disease material CM334 as the test material to clone and obtain the transcription factor CaWRKY14 in response to Phytophthora infestans. Bioinformatic analysis showed that the gene has a total DNA length of 2 530 bp and a cDNA length of 1 662 bp. It contains 5 introns, encodes 553 amino acids, and contains a WRKY conserved domain. Realtime fluorescence quantitative expression analysis showed that CaWRKY14 was not only induced by ABA and Phytophthora capsici stress, the expression level reached the peak at 12 h and 24 h, respectively, 8.54 and 8.04 times of the control. It is also induced by high salt, heat shock and drought stress. In addition, after silencing CaWRKY14 transcription factor using VIGS technology, it was found that the diseaseresistant material CM334 tended to develop disease after inoculation with Phytophthora infestans. Studies have shown that CaWRKY14 gene may play an important role in the process of pepper response to Phytophthora capsici.