The lysophosphatidic acid acyltransferase (LPAT) family genes were screened and identified from the transcriptome database of perilla. The sequence characteristics and protein structures of the family genes were analyzed using bioinformatics methods and the spatiotemporal expression characteristics of genes were studied using qRTPCR technology. The research provides a basis for better understanding the mechanism of perilla oil synthesis. The results showed that: (1) a total of 11 LPAT family genes were detected, including PfLPAT1, PfLPAT21, PfLPAT22, PfLPAT23, PfLPAT24, PfLPAT41, PfLPAT42, PfLPAT51, PfLPAT52, PfLPAT53 and PfLPAT54. The length of amino acids encoded by PfLPATs genes ranged from 250 to 384 aa, and the theoretical isoelectric point varied from 7.6 to 9.6. (2) The analysis of gene sequence comparison showed that 11 PfLPATs proteins belonged to three subclasses, respectively, among which type 1 LPAT contained one gene, type 2/3 LPAT contained four genes and type 4/5 LPAT contained six genes. (3)Realtime fluorescent quantitative PCR results indicated that 11 LPAT family genes were expressed in different tissues of ‘Jin Zisu 1’. Among them, LPAT21, LPAT22 and LPAT23 expressed highly in seeds. It was speculated that they play an key role in the process of perilla seed oil synthesis and metabolism. The results of this study provided important genetic information for further study on the functions of LPAT family genes.