According to the data of Sol Genomic Network, we obtained the fulllength of SlWRKY6 (Solyc02g080890) from cultivated tomato variety M82 by RTPCR and verified its biological function by prokaryotic expression. The results showed that: (1) an open reading frame (ORF) of SlWRKY6 was obtained from Solanum lycopersicum, which is 1 653 bp long and encodes 550 putative amino acids with a WRKYGQK conserved domain and C₂H₂ zinc finger domain, belonging to IIb subgroup. The upstream of the SlWRKY6 promoter contains several hormone response elements and abiotic stress response elements. (2) Phylogenetic tree analysis showed that SlWRKY6 shared a high degree of sequence similarity with SpWRKY31X1 (NP_001352691.1) from Solanum pennellii and localized to the nucleus. (3) qRTPCR results showed that the pattern of SlWRKY6 was highly expressed in leaves and also induced by salt and drought stress. (4) SDSPAGE and Western blot analysis showed that the molecular weight of SlWRKY6 was approximately 66 kDa. (5) Prokaryotic expression analysis showed that the growth rate of recombinant E. coli BL21∷pET30aSlWRKY6 was significantly lower than that of the control E. coli BL21∷pET30a under different concentrations of salt (NaCl) or drought (mannitol) stress, especially under 400 mmol/L NaCl and 800 mmol/L mannitol stress. In addition, the drip plate test showed that SlWRKY6 gene could enhance the tolerance of E. coil to salt, drought and ABA stresses. Taken together, the transcription factor SlWRKY6 might respond to abiotic stress by participating in the ABA pathway.