SWEET (sugar will eventually be exported transporters) protein participates in the loading of phloem sugars. In this study, 9 BeSWEET sequences were screened based on the transcriptome library of Bambusa emeiensis and analyzed by bioinformatics; Genes of BeSWEET42 and BeSWEET1a2 among the 9 BeSWEET sequences were cloned with the cDNA of the stem and young leaf of B. emeiensis. Real time quantitative expression analysis (qRTPCR) was used to analyze its expression levels in mesophyll, veins, roots, stems and the expression changes of BeSWEET gene induced by exogenous sugar. The results showed that: (1) phylogenetic tree analysis showed that 9 BeSWEET sequences were divided into four clades. BeSWEET42 which has two MtN3 conserved domains is homologous to rice SWEET4, clustered into Clade Ⅱ. BeSWEET1a2 is homologous to rice SWEET1a and SWEET1b, and clustered into Clade Ⅰ. (2) Sequence analysis showed that BeSWEET42 and BeSWEET1a2 encode 256 and 222 amino acids, respectively, and have 7 and 5 transmembrane domains, respectively. (3) The prediction of subcellular localization indicates that both genes are located in the plasma membrane. (4) qRTPCR analysis shows that BeSWEET42 and BeSWEET1a2 are mainly expressed in roots and veins, respectively. It is speculated that they may cooperate in the transport of carbohydrates from source to sink. (5) Both BeSWEET42 and BeSWEET1a2 were significantly upregulated under the induction of hexose, showing a preference for hexose. Our study provides the insight for understanding of biological functions of B. emeiensis BeSWEET proteins in regulating sugar transport.