UDPflavonoid glycosyltransferase (UFGT) catalyzes the formation of stable glycosides such as flavonols and anthocyanins, which is the key to the last step of the synthesis of flavonol and anthocyanin glycosides. In this study, we used the petals of Camellia nitidissima Chi as materials, cloned two UDPflavonoid glycosyltransferase genes selected from the transcription by PCR amplification. The results show that: (1) CnUFGT14 gene, with GenBank numbers MT370521, was 1 562 bp in length, 1 380 bp in open reading frame, and encoded 459 amino acids. CnUFGT15 gene, with GenBank numbers MT370520, was 1 546 bp in length, 1 368 bp in open reading frame, and encoded 455 amino acids. Both protein sequences had a conserved region of PSPG characteristic of UFGT proteins. (2) Phylogenetic tree analysis showed that CnUFGT14 and CnUFGT15 were closely related to UFGT78A14 and UFGT78A15 in C. sinensis, respectively. (3) Quantitative PCR analysis showed that the expression levels of CnUFGT14 were positively correlated with the contents of multiple polyphenol components, while CnUFGT15 expression levels were not significantly correlated with the contents of flavonols and polyphenols. (4) The subcellular localizations showed that CnUFGT14 and CnUFGT15 proteins were in the nuclear membrane, cytoplasm and cell membrane. (5) The transform of tobacco with leaf disc method found that the total polyphenol content and the contents of multiple polyphenol components in the plants with high expression level of CnUFGT14 increased, while the changes of flavonoids and polyphenol components in the transgenic lines of CnUFGT15 were not significant. All the results indicated that CnUFGT14 gene can promote the synthesis of polyphenols and CnUFGT15 had no obvious effect on the flavonoid pathway.