TCP genes encode a plantspecific transcription factor family, which acts as a key role in regulating stress response． In this study, the members of TCP genes family were identified in the whole genome of strawberry by bioinformatics analysis, the qRTPCR was used to detect the expression of strawberry transcription factor family in abiotic stress, while gene structure and function of its corresponding transcription factor family genes were analyzed, to provide a foundation for exploring the role of TCP transcription factor in abiotic stress of Fragaria vesca. The results showed: (1) 18 strawberry genes (FvTCP) were identified from F. vesca genome and 58 strawberry genes (FaTCP) were also identified from F. ananassa genome. According to the subcellular localization，the TCP family genes were mainly located in the nucleus. (2) qRTPCR showed that the family genes had response to adversity stress effect, but different members response under different stress level. FvTCP14 tendency in 200 mmol·L^-1 NaCl, 10% PEG, 100 μmol·L^-1 ABA, 4 ℃, 40 ℃ and 100 mmol·L^-1 H₂O₂ treatments were significantly upregulated, which were 43.78, 166.73, 38.39, 265.87, 626.24 and 451.85 times of the control, respectively which indicated that FvTCP14 strongly responded to drought stress, salt stress, ABA induction, H₂O₂, high and low temperature stresses. In addition, FvTCP12 were significantly downregulated after 4 ℃, 100 μmol·L^-1 ABA and 100 mmol·L^-1 H₂O₂ stress treatments, suggesting that this gene had a negative regulatory effect on low temperature, ABA and H₂O₂ stresses. It is found that the expression of FvTCPs were different when the encountered the adversity environments.