Phloem unloading and postphloem transport play pivotal parts in the regulation of the distribution and accumulation of photosynthate in the fruit， and largely determine the yield and quality of the crop． To uncover the phloem unloading and transport pathway of photosynthates in Ziziphus jujuba Mill. cv. lingwuchangzao fruit, the four different developmental periods fruits of Ziziphus jujuba Mill. cv. lingwuchangzao were used as experimental material to observe the microstructure of the vascular bundle of fruit in each development period, the techniques of fluorescent dye tracer in living cell and confocal laser scanning microscopy (CLSM) were used to observe the phloem unloading path of photosynthates in the fruit timely, the research results provide scientific bases of fruit photosynthates accumulation and quality regulation for Ziziphus jujuba Mill cv. lingwuchangzao． The main research results were followed: (1) not only the phloem of the fruit but also the parenchyma cells surrounding the phloem existed evidently the CF green fluorescence, the SECC complex and its surrounding cells existed symplastic integration in the early bulking period. (2) The CF green fluorescence was mainly confined to the phloem of the fruit, there is less CF green fluorescence in the parenchyma cells surrounding the phloem, the SECC complex and its surrounding cells existed mainly the symplastic isolation but existed few symplastic integration in the rapid enlargement period. (3) The CF green fluorescence was confined to the phloem of the fruit, and there was no CF green fluorescence in the parenchyma cells surrounding the phloem in the coloring period and the maturation period, the SECC complex and its surrounding cells existed entirely the symplastic isolation, the CF green fluorescence in the parenchyma cells surrounding the phloem increased significantly while leading in CFDA and Digitonin with penetrating plasma membrane function. Research suggests that the photoassimilates unloading from the phloem was symplast pathway in the early bulking period fruit, the photoassimilates unloading and transport adopted mainly apoplastic pathway in the rapid enlargement period, but existed simultaneously symplast pathway, the photoassimilates unloading and transport adopted apoplastic pathway in the coloring period and the maturation period fruit.