Calmodulinlike protein (CML) is a kind of important Ca²⁺ binding protein in plants, which plays a critical role in plant growth, development, and stress response. In this study, StCML gene family members were identified in potato genome by bioinformatics methods, and their expression patterns and stress response were analyzed, which laid a theoretical foundation for further understanding the mechanism of StCML gene family members in growth and development and stress response. The results showed that: (1) a total of 80 StCML genes were identified from the Solanum tuberosum L. genome, and all of them have the EFhand domains. According to the topological structure of phylogenetic tree, they were divided into 5 subfamilies, each of which has respectively 18, 12, 14, 12, and 24 members, and most of the members have relatively conservative gene structures and motifs; (2) Through the analysis of RNASeq data, it was found that StCML genes showed tissuespecific expression in flowers, petioles, shoots, stamens, stolons and tubers, they were mainly responded to salt, heat, drought stress and GA₃. (3) qRTPCR analysis showed StCML13, StCML21 and StCML53 were upregulated under cold stress, StCML11, StCML21 and StCML39 were upregulated under heat stress, StCML21 and StCML60 were upregulated under salt stress, StCML53 was upregulated under Ralstonia solanacearum infection, indicating that StCML family is able to response to multiple stresses. These results could serve as the foundation for indepth research on the mechanism of the StCML gene family regulating plant stress tolerance.