In this study, the species diversity and geographical distribution pattern of wild Impatiens in Guizhou Province were studied based on the establishment of a list and geographical distribution database of wild Impatiens in Guizhou Province. Through the literature and a field investigation, we analyzed the species composition, endemism, horizontal distribution, vertical distribution and similarity. Finally, the hotspots of Impatiens in Guizhou were determined by a complementary algorithm，This study is of great significance to the conservation and sustainable utilization of Impatiens plant resources in Guizhou Province. The results show that: (1) there are 60 species (including subspecies) of Impatiens in Guizhou Province. These plants are distributed among 69 countylevel administrative districts. There are 55 species endemic to China and 10 endemic to Guizhou, and the vertical distribution is the most abundant at altitudes of 1 000~1 500 m. (2) The calculation of the provincial similarity coefficient showed that Guizhou and Hunan had the highest similarity. Finally, 9 hot spots were selected, representing 86.67% of the Impatiens plants. (3) Guizhou Province has a warm and humid climate with typical karst landforms, which have abundant Impatiens plant and have high species diversity and regional specificity.