Present study used the inflorescences of six grape varieties with different fruit shapes as the experimental materials. The expression of VvIQD10, a member of the grape IQ67 domain (IQD) gene family, was compared by fluorescence quantitative PCR analysis. The coding region sequence of the VvIQD10 gene was cloned from the oval grape variety ‘Tianshan’ and analyzed by bioinformatics. The subcellular location transient expression vector was used to transform tobacco to study the distribution of VvIQD10 protein in the cell. Yeast2hybrid assay and bimolecular fluorescence complementation test were used to study protein interaction. The results showed that: (1) the expression level of VvIQD10 gene in longshaped grape varieties was higher than that of nearly round grape varieties. (2) The length of the open reading frame of the VvIQD10 gene was 1 401 bp, which encoded 466 amino acids. (3) VvIQD10 protein was an unstable hydrophilic protein with no signal peptide and transmembrane domain, but it contained a conserved IQ67 domain. The main structure was alpha helix and random coil. It was closely related to a member of the kiwi IQD family (PSS09955.1). (4) VvIQD10 protein is mainly located in microtubules and plasma membranes, and directly interacted with cytoskeletal tissuerelated proteinscalmodulinlike proteins (VvCML). Studies suggested that the VvIQD10 gene may be involved in the regulation of grape fruit shape, and the recruitment of VvCML protein from cytoplasm to microtubules depended on VvIQD10. It is speculated that VvIQD10 protein may regulate cytoskeletal movement by combining with calmodulinlike proteins, and then participate in shaping of grape berries.