This study used the MTP sequence of model species as seed sequence to BLASTP the amino acid sequence database of cucumber, and then systematically analyzed the features of obtained CsMTP candidates. To analysis the expression pattern of CsMTPs, qRTPCR analysis were performed using cucumber leaf and root tissues treated by Zn²⁺, Cd²⁺, Mn²⁺, Cu²⁺, Fe²⁺ and Mo²⁺ stresses, which will provide theoretical basis for further studies related to the regulation mechanisms of CsMTPs in cucumber under heavy metal stresses. The results showed that 10 CsMTPs can be divided into Fe / ZnCDF (2) MnCDF (3) and ZnCDF subfamily (5), which were further named as CsMTP3 to CsMTP12 based on phylogenetic relationship. Most CsMTPs contain 6 transmembrane domains (TMD). CsMTP8, CsMTP9 and CsMTP11, which belong to MnCDF subgroup, contain the same sequence DxxxD, HxxxD motifs were observed in CsMTP3, 4, 5 and CsMTP7 of ZnMTP and Fe/Zn CDF subgroups. RNAseq analysis revealed that CsMTPs showed tissue and treatmentspecific expression patterns. CsMTP11, CsMTP3 and CsMTP7 maintained relatively higher expression level and exogenous chemical reagents can induce different expression patterns of the same gene. (3) qRTPCR study demonstrated that CsMTPs could be induced by the treatment of different metals, including metals that are not the potential substrate of MTP family.