In this study, based on the transcriptome results and genome database of mulberry, we cloned the cDNA and promoter sequence of MaPP2C8 using PCR method. Bioinformatics methods were used to analyze the cDNA and promoter sequence of MaPP2C8 and the expression pattern of MaPP2C8 under drought treatment was determined by using qRTPCR method. The results show that: (1) the fulllength cDNA of MaPP2C8 gene was 1 309 bp，which contained an opening reading frame of 1 053 bp and encoded a protein of 350 amino acids residues. (2) MaPP2C8 protein was highly homologous with the species in Moraceae and belongs to the A branch in the PP2Cs family. (3) MaPP2C8 protein may locate in multipositions of the cell, such as the nucleus, cytoplasm, cytomembrane, etc. (4) A 1 612 bp promoter fragment upstream of translation initiation site was isolated from mulberry. MaPP2C8 promoter contained three types of hormonerelated cisacting elements, and as many as 3 elements associated with ABA. (5) The expression of MaPP2C8 gene was upregulated by drought treatment, and its expression level was significantly downregulated after rewatering treatment. Our studies suggest that MaPP2C8 gene may play an important role in response to drought stress in mulberry.