The nodulin 26like intrinsic proteins (NIPs) are a subclass of aquaporin, which plays an important role in the process of plant nutrient acquisition and stress response. In this study, we used a variety of bioinformatics software to conduct bioinformatics analysis of grape NIP family genes, and cloned four VvNIP genes by reverse transcription PCR. We used qRTPCR to analyze the expression characteristics of NIP genes under abiotic stress. The results showed: (1) a total of 8 NIP genes were identified in the grape genome and they were distributed on the 4 chromosomes of grapes, mainly located in the plasma membrane. The structure contains 6 transmembrane domains and two typical conserved NPA domains. Besides, there were many possible phosphorylation sites in the amino acid sequences. (2) Evolutionary analysis showed that the NIP genes of grape and Arabidopsis thaliana had high homology, the gene structure contained 4-6 gene structures with exons and the type and quantity of gene conserved sequences were similar. The 2 kb upstream of the gene promoter contains a variety of cisregulatory elements that responded to stress and hormones, the quantity difference may be related to the function of gene itself. (3) The expression levels of NIP family genes in different tissues were quite different. Most members had higher expression levels in leaves, and lower expression levels in stems. In addition, four VvNIP genes were cloned and their lengths were 789 bp, 606 bp, 897 bp and 789 bp, encoding 262, 201, 298 and 293 amino acids. (4) qRTPCR results showed that the expression levels of NIP genes in grape leaves were different under different stress treatments: most of the grape NIP genes were significantly downregulated under low temperature treatment; under salt stress, the other family genes except VvNIP21 and VvNIP42 were downregulated; VvNIP42 was significantly upregulated under drought stress. The results showed that VvNIP genes were responsive to multiple stresses, it provides a reference for the study of grape stress mechanism.