Cloning and Expression Analysis of LbCAT and LbGPX in Lilium
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    Abstract:

    Catalase gene (LbCAT) and glutathione peroxidase gene (LbGPX) were cloned from cutting lily (Lilium brownii var. viridulum)‘Corvara’cDNA. Sequence analysis showed that the two genes contained 1 479 bp and 519 bp, the opening reading frames (ORFs) were 492 and 172 amino acids, respectively. The evolutionary analysis showed that the amino acid sequences of LbCAT protein and CAT protein of Lilium regale had the highest consistency (99.19%) and the closest genetic relationship. The amino acid sequences of LbGPX protein and oil palm GPX protein had the highest consistency (78.61%) and the closest genetic relationship. The results of expression showed that LbCAT and LbGPX genes were transcribed in roots, bulbs, leaves and flowers. The leaves had the highest transcript profile of LbCAT and the flowers had the highest expression levels of LbGPX. These two genes were transcribed in different stages of lily buds and the expression levels increased following the buds growing . After PEG treatments, the transcription levels of the two genes surged, while the SLs treatments significantly lowed the two genes expression levels. These results provided a certain basis for the study and breeding of lily stress resistance.

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CHI Bowen, LIU Li, XIE Tian, YAN Junhui, ZHAO Zixian, WEN Jinfen. Cloning and Expression Analysis of LbCAT and LbGPX in Lilium[J]. Acta Botanica Boreali-Occidentalia Sinica,2021,41(9):1467-1474

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  • Online: September 28,2021
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