Megasporogenesis, microsporogenesis and the development of female and male gametophyte of Clivia miniata Regel were studied using normal paraffin method in this paper. The results enriched C. miniata reproductive biological data and provided theoretical basis for the cultivation. In order to explore the anatomical characteristics of reproductive biology of C. miniata and provide theoretical basis for the cultivation of C. miniata. The main results are as follows: (1) C. miniata has four anther chambers and the tapetum is of secretory type. (2) Cytokinesis of microsporocyte meiotic division is of successive type, tetrad is bilatera and the mature pollen grains are twocelled. (3) The ovule is anatropous double integuments and crassinucellate. Embryo sac is of polygonum type. (4) The corresponding relationship between male and female gametophyte development was recorded, and it was found that male gametophyte development tended to synchronize and female gametophyte development was not synchronized. (5) The female gametophyte is still in the stage of tetraconucleus and octagonucleus embryo sac when the pollen began to spread. At the stage of mature embryo sac, the two polar nuclei of the central cell are located at the end of the antipodal cell, and the antipodal cell is degraded. There has a hypostase above the chalazal end.