In this study, the stems of Cinnamomum migao with axillary buds were used as experimental materials to establish an induction and multiplication culture system for C. migaos adventitious buds. It was explored that the influence of adventitious bud induction and proliferation of C. migao stems with buds under the conditions of different disinfection time, sampling time, and different hormone types and concentration ratios. The results showed that: (1) the most suitable disinfection method for explants was 75% alcohol for 30 s + 0.1% HgCl₂ for 6.5 min, and November was the best time for explants to be disinfected. (2) The most suitable adventitious bud induction medium is MS + 3.0 mg/L 6BA + 0.3 mg/L IBA + 6.5 g/L agar + 30 g/L sucrose, the adventitious bud induction rate reaches 81.24%; both 6BA and NAA can promote C. migao for the proliferation of adventitious buds, NAA is the main factor influencing the proliferation of adventitious buds. (3) The most suitable adventitious bud multiplication medium is MS + 0.1 mg/L 6BA + 1.0 mg/L NAA + 0.3 g/L AC + 6.5 g/L agar + 30 g/L sucrose, and the average adventitious bud multiplication coefficient reaches 2.79. This study basically established the adventitious bud induction and multiplication culture system of C. migao, which provides a new way for its indepth resource development and utilization.