(1) To investigate the relationship between sulfate transporter genes (SULTR) and βNoxalylLα,βdiaminopropionicacid (βODAP) content in Lathyrus sativus, we first identified 13 LsSULTR genes from transcriptomic database (SRP145030), which coding the four major groups of SULTR Ⅰ-Ⅳ separately. Therein, LsSULTR3; 3 and LsSULTR3; 5 were characterized by the conversed STAS (PF01740) and Sulfate_transp (PF00916) domain of SULTR, and gene expression levels of LsSULTR3; 3 and LsSULTR3; 5 strongly suggested relation with βODAP biosynthesis. Bioinformatics analysis suggested that LsSULTR3; 3 and LsSULTR3; 5 can be regulated by various cisacting elements such as MYB and hormone response factors. In addition, proteinprotein interactions and protein phosphorylation also play an important role. (2) cDNAs of LsSULTR3; 3 and LsSULTR3; 5 were cloned with 1 962 bp encoding a peptide of 653 amino acid residues, and 1 923 bp encoding a peptide of 640 amino acid residues, respectively. (3) Semiquantity RTPCR analysis showed that LsSULTR3; 3 gene was expressed highest in stems and followed by main roots, old leaves, flowers, seeds in early flourishing podding stage (S2) and seedfillingflourishing stage (S6); while the LsSULTR3; 5 gene was expressed highest in flowers and followed by main roots, lateral roots and seeds in S2 stage. These results will help to reveal the mechanism of sulfur transport and assimilation, βODAP biosynthesis in L. sativus.