Abstract:In order to understand the change characteristics of soil bacterial community structure and soil physical and chemical properties caused by land uses in salinized soil, this study selected CK (Original salinized soil0, PD12Y [Continuous rotation of rice and corn for 12 years (corn)], P12Y (Rice continuous cultivation for 12 years), DP12Y [Continuous rotation of rice and corn for 12 years (rice)], D12Y (Continuous corn for 12 years). Illumina HiSeq highthroughput sequencing technology was used to analyze the characteristics of soil bacterial community structure in different planting years of maize (D12Y), and the relationship between soil physical and chemical factors and bacterial community structure was analyzed. The results showed that: (1) proteobacteria was the most dominant flora in all soils, accounting for 21.25%-46.87%. The second most dominant bacteria group was Blastomonas, accounting for 7.10%-25.36%. Its abundance in the salinized wastelands (CK) treatment was 25.36%, 96.28%-257.18% higher than that in other treatments (P<0.05). The third most dominant phyla were Actinobacteria, accounting for 5.30%-18.87%, and the relative abundance of Actinobacteria was the highest in CK, 105.00%-261.51% higher than other treatments (P<0.05). (2) At the genus level, Salinimicrobium, unidentified Actinomarinales and Candidatus entotheonella of salttolerant soils were significantly decreased by different land use salinization methods (P<0.05). (3) The results of soil physical and chemical properties showed that total nitrogen, alkalihydrolyzed nitrogen and total phosphorus were the highest in PD12Y and D12Y treatments, and alkalihydrolyzed nitrogen in D12Y treatment was significantly higher than that in other treatments (P<0.05), and total P in PD12Y treatment was the highest (P<0.05). The available P in PD12Y treatment was significantly higher than that in other treatments (P<0.05). At the same time, soil pH (P<0.05), electrical conductivity and water content were the lowest in PD12Y treatment. (4) Redundancy analysis (dbRDA) and Spearman correlation analysis showed that soil water content and total nitrogen were the main factors affecting the bacterial community structure.