SAUR (small auxin up RNA) is one of the main auxin inducing genes in plants, which plays an important regulatory role in promoting plant cell division and cell elongation. In this study, Impatiens uliginosa Franch. was used as material, and the SAUR gene was cloned by RTPCR and analyzed by bioinformatics and gene expression. The results showed that the cDNA sequences of the six SAUR genes were 351, 534, 396, 333, 309 and 411 bp, encoding 116, 177, 131, 110, 102 and 136 amino acids, respectively. Bioinformatics analysis showed that SAUR3 was stable, and the other five proteins were unstable. All the proteins were hydrophilic except SAUR5 which was hydrophobic. All proteins are nonsecretory proteins. The amino acid sequences encoded by these six SAUR genes had higher homology with other plants, and showed certain conserved characteristics of species. It is speculated that SAUR4 and SAUR5 are paratellate relatives, and SAUR1, SAUR2, SAUR3 and SAUR6 are direct relatives.RtPCR analysis showed that the relative expression levels of the six SAUR genes were the highest at the initial flowering stage, followed by the full flowering stage, and the lowest at the bud stage. SAUR3 gene was highly expressed in both spur and blade. SAUR4, SAUR5 and SAUR6 genes were highly expressed in the basel and blade of p. I. uliginosa Franch, while the other three SAUR genes were not significantly expressed in the tip and camber of I. uliginosa Franch. This study provided some basic data and scientific basis for the regulation mechanism of flower spacing, flower pattern improvement and new variety breeding of Impatiens.