In order to clarify the influence mechanism of light quality on the growth of Loropetalum chinense var. rubrum callus and the synthesis and accumulation of secondary metabolites flavonoids, to provide a theoretical basis for manmade regulation of the yield and quality of its cell products, we used, the callus induced by L. chinense var. rubrum leaves as the test material in this experiment with the darkness (CK) as control. White light (W), red light (R), blue light (B), blueviolet light (BP), and blue light + UVA (B+UVA) and UVA treat for 15 and 30 d, respectively. Through comparative analysis of callus growth, physiological indicators, anthocyanin and total flavonoids content, we explored the effect of light quality on L. chinense var. rubrum callus growth and flavonoid content. The results show that: (1) compared with CK, several different light quality treatments can increase the dry and fresh weight of callus. Among them, R is more conducive to the increase of callus fresh weight, B+UVA and UVA are more conducive to the increase of dry weight, and there are some genotypic differences in the influence of light quality on the dry weight of callus. (2) R, B and W treatments can significantly increase the solubility of L. chinense var. rubrum callus sugar content, among which R treatment has the highest valueadded, B and W treatments second, and other shortwave treatments slightly increase or decrease compared with 0 d. (3) Shortwavelength light quality treatment significantly improves the callus of L. chinense var. rubrum. Among them, B+UVA treatment has the highest valueadded content. (4) Shortwavelength light quality treatment is more conducive to increasing the PAL activity of L. chinense var. rubrum callus. Among them, BP and B+UVA treatments are the most significant. (5) Shortwavelength light quality (B, BP, B+UVA and UVA) can promote the synthesis of anthocyanins and flavonoids in the callus of L. chinense var. rubrum, while R treatment play an inhibitory role. It was shown that shortwave light quality B, BP, B+UVA and UVA treatments can promote the synthesis of anthocyanins in the callus of L. chinense, B+UVA and B treatments are more conducive to the increase of total flavonoids content; Moreover, there are certain genotypic differences in the influence of light quality on the dry weight of callus, anthocyanin and total flavonoids.