Seven treatments including control (CK), average fertilization (AF1, AF2), exponential fertilization (EF1, EF2, EF3 and EF4) were applied to the oneyearold container seedlings of Taxus madia × T. yunnanensis ‘Yunman’(YM). The active component 10DAB, growth, shoot biomass and 10DAB accumulation, as well as the N, P and K accumulation in different organs were measured under different fertilization methods and rates. The effects of different fertilization methods and fertilization amounts on 10DAB and nutrient accumulation of YM seedlings were studied. The results provide a theoretical and practical basis for precise regulation of nutrients and efficient cultivation of YM in seedling stage. The results showed that: (1) fertilization significantly increased the content of 10DAB in the branches and leaves of seedlings and promoted the growth and biomass accumulation of branches and leaves (P<0.05). Under the same nitrogen application rate, the branch and leaf biomass of exponential fertilization was significantly higher than that of average fertilization (P<0.05). The content of 10DAB in branches and leaves was higher in EF2 and EF3 treatments, and the growth and branch and leaf biomass were the largest in EF2 treatment. (2) The accumulation of 10DAB in exponential fertilization EF2 treatment was significantly higher than that in other treatments (P<0.05). The order was EF2 > EF1 > AF2 > EF3 > AF1 > EF4 > CK. Exponential fertilization EF2 was 30.61%-41.94% higher than average fertilization AF1 and 18.14%-25.00% higher than average fertilization AF2. (3) The effects of fertilization on the contents of N, P and K in all parts of seedlings reached a significant level (P<0.05). Under the same nitrogen application rate, the contents of N, P and K in each part of exponential fertilization were better than that of average fertilization. In exponential fertilization, the N content in roots and K content in each part increased with the increase of fertilization, while the N content in stems and leaves and P content in all parts, first increased and then decreased with the increase of fertilization. The distribution ratio of N, P and K showed the rule of leaf > root > stem. N and P, P and K in all parts of seedlings and N and K in roots and leaves showed significant (P<0.05) positive correlation, which was a synergistic relationship. (4) The accumulation of 10DAB in YM is the highest from September to October. At this time, the accumulation of 10DAB available for harvesting is the highest and the income is the largest. The study found that compared with conventional fertilization, exponential fertilization could increase the contents of 10DAB and the accumulation of nutrients in YM seedlings, and the amount of fertilizer applied was 1 600 mg·plant^-1.