SPL exists in all green plants and plays an important role in plant growth and leaf formation. In this study, ‘Gailiangxianggu’ Chinese kale was used as material, and the whole genome of BoSPL family was identified and analyzed by bioinformatics method. The genes BoSPL3-1, BoSPL10-2 and BoSPL11-2 were cloned by PCR, and the quantitative analysis of different growth stages and different tissue parts of Chinese kale was carried out, which laid a foundation for revealing the molecular mechanism of the leaf development process of ‘Gailiangxianggu’ Chinese kale and cultivating new varieties of Chinese kale. The results show that: (1) there are 31 members of BoSPL family, and the amino acid length of the coding region proteins are 157-1 028 aa. The amino acid of BoSPL protein has no signal peptide, which belongs to non-secretory protein; The results of subcellular localization prediction showed that all BoSPL family members were located in the nucleus, and a few members were located in vacuoles, chloroplasts and cytoplasm. BoSPL family genes contain 1-9 introns, and all members contain SBP conserved domains. Phylogenetic analysis shows that BoSPL can be divided into 8 subgroups of evolutionary groups, with different number of genes in each group, and the evolutionary relationship of BoSPL is similar to AtSPL. (2) Successfully cloned the BoSPL3-1, BoSPL10-2 and BoSPL11-2 genes of Chinese kale, and successfully constructed pCAMBIA1302-35S-BoSPL3-1-GFP, pCAMBIA1302-35S-BoSPL10-2-GFP and pCAMBIA1302-35S-BoSPL11-2-GFP. Transient transformation of tobacco lower epidermal cells mediated by agrobacterium tumefaciens GV3101, and laser confocal microscopy showed that BoSPL3-1, BoSPL10-2 and BoSPL11-2 were all located in the nucleus, which was consistent with the predicted results. (3) qRT-PCR analysis showed that the relative expression levels of BoSPL3-1, BoSPL10-2 and BoSPL11-2 were all higher in the leaves of ‘Gailiangxianggu’ Chinese kale, but there was no significant difference in the relative expression levels of the three genes in mesophyll, while BoSPL10-2 was significantly higher in the flower-shaped deformed leaves, which might be related to the formation of flower-shaped deformed leaves.