WUSCHEL-related homeobox (WOX) transcription factors are plant-specific transcription factors that can regulate plant growth and development and the response to abiotic stress. In this study, the GuWOX gene family was analyzed by bioinformatics methods, the qRT-PCR method was used to detect the expression of this gene family in different tissues and abiotic stress, and four GuWOX genes were cloned by RT-PCR method. The results showed that: (1) there were a total of 16 GuWOX genes, all of which contained a conserved HD domain and were hydrophilic and unstable proteins. Phylogenetic tree analysis showed that the GuWOX family was divided into 3 major clades (e.g., the Ancient clade, the Intermediate clade and the Modern/WUS clade). Numerous stress and hormone-responsive cis-elements were observed in the GuWOX promoter regions. (2) qRT-PCR showed that the expression of GuWOX1 was the highest in lateral roots and GuWOX15 was the highest in stems. At different time intervals, GuWOX was expressed under salt, phosphorus free and GA₃ treatments. The expression pattern of GuWOX15 was similar under drought stress and ABA treatment, and the expression was up-regulated at 12 h. (3) GuWOX1, GuWOX5, GuWOX6, GuWOX12 genes were cloned and their lengths were 557 bp, 707 bp, 716 bp, 578 bp, encoding 184, 237, 237, 191 amino acids, respectively. The results of this study indicate that GuWOX genes were involved in the development of G. uralensis, the response to adversity stresses, and the regulation of plant hormones. This result laid the foundation for further analysis of GuWOX gene function.