Cassava (Manihot esculenta Crantz) is an important food crop and cash crop in tropical and subtropical regions. UDPglycosyltransferases (UGTs) belongs to glycosyltransferases, which is found in all plant species. In this research, Manihot esculenta cv. SC124 was regarded as material, and the MeUGT41 gene was cloned by RTPCR and its function in cassava bacterial blight resistance was analyzed. These research provided clues for further study on the mechanism of MeUGT41 gene in cassava resistance to biotic stress. The results showed that: (1) the expression of MeUGT41 gene was significantly induced by Xamthomonas axonopodis pv. Manihotis (Xam). (2) Virus induced gene silencing (VIGS) vector of MeUGT41 gene was constructed. Through gene silencing in cassava leaves, qRTPCR results showed that MeUGT41 gene was significantly decreased in cassava leaves. (3) Subsequently, Xam infection experiments were carried out on cassava leaves, and the results showed that the downregulation of MeUGT41 gene expression significantly reduced the resistance to Xam infection.