NADPmalate enzyme (NADPME) is a key photosynthetic enzyme in C₄ plants and plays an important role in biotic and abiotic stress. To further study the function of NADPME, we cloned the NADPME family gene sequences from the leaves of C₃C₄ species Salsola laricifolia, and then determined the expression patterns in tissues under different abiotic stresses. We also cloned NADPMEs promoter sequences and analyzed the element differences in response to abiotic stress based on bioinformatics software. The results showed that: (1) three NADPMEs were obtained, named SaNADPME1, SaNADPME2 and SaNADPME4, with the length of CDS sequence was 1 755 bp, 1 758 bp and 1 941 bp, respectively. (2) SaNADPME1 was mainly expressed in roots, while SaNADPME2 and SaNADPME4 were mainly expressed in leaves. Under the stress of ABA, NaCl, NaHCO₃ and PEG₆₀₀₀, three NADPMEs could be induced to express in Salsola laricifolia, and the response expression patterns of SaNADPME4 and SaNADPME2 were similar. (3) The length of promoter regions of SaNADPME1, SaNADPME2, and SaNADPME4 were 2 351 bp, 1 655 bp and 2 887 bp. Bioinformatics analysis showed that they contained not only basic promoter elements, but also elements in response to stress response.