In order to explore the influencing factors of the establishment of regeneration system of Gymnocarpos przewalskii and determine the origin of adventitious shoots, with the stem segments of the superior individual of G. przewalskii as explants, we used different concentrations of 6  BA and IBA to screen the optimal concentration combinations for callus proliferation and adventitious shoot regeneration in this study. The key influencing factors for rooting induction were determined, and the regeneration system was established. The differentiation process of adventitious shoots was analyzed to confirm its origin. The results showed that : (1) the optimal medium for callus proliferation was MS+1 mg·L^-1 IBA+1 mg·L^-1 6BA+30 g·L^-1 sucrose+7 g·L^-1 agar. Intersubjectivity analysis showed that IBA was the key influencing factor. The callus size increased first and then decreased with the change of IBA concentration. (2) The best adventitious shoot induction medium was MS+0.5 mg·L^-1 6BA+30 g·L^-1 sucrose+7 g·L^-1 agar, the number of adventitious shoots was 4.9 per piece, and the germination rate was 92.3%. (3) For rooting induction, SH basic medium and sucrose concentration were the key factors. The optimal rooting medium was SH+0-10 g·L^-1 sucrose+7 g·L^-1 agar, and the rooting rate was 91.3%. (4) Adventitious shoots originated from meristem cells on the surface of callus, which were exogenous. In this study, the regeneration system of G. przewalskii was established through organogenesis, and the adventitious shoots were identified as the external origin, which laid a foundation for the protection and sustainable utilization of G. przewalskii, a rare and endangered forest germplasm resource, and provided an effective way for its future development and utilization.