MYB transcription factor family is widely involved in plant response to abiotic stresses, including drought, salinity, and chilling. To further study MYBs in okra ［Abelmoschus esculentus (L.) Moench］, in this study, we cloned a gene of AeMYB1R1 from ‘Hokkaido No.1’ of okra by PCR and characterized using bioinformatic analysis. Quantitative realtime PCR (qRTPCR) was used to analyze AeMYB1R1 expression pattern and the expression characterization under abiotic stresses. The results showed that: (1) AeMYB1R1 was successfully cloned from okra, which contained an open reading frame of 1 056 bp, encoding 352 amino acids. Sequence alignment and phylogenetic tree analysis showed that, AeMYB1R1 was evolutionarily highly conserved in plants. AeMYB1R1 had a molecular weight of 37 891.57 Da and an isoelectric point of 8.75, which contained more glutamic acid and less tryptophan, as well as more potential phosphorylation sites and glycosylation sites. (2) Structural analysis showed that AeMYB1R1 was mainly dominated by αhelix and random coil, without signal peptide and transmembrane structure, which was a hydrophobic protein. Meanwhile, its amino acid sequence contained a conserved domain from 104 to 156, indicating that it belonged to the SHAQKYF class of MYBs. (3) qRTPCR results showed that AeMYB1R1 had the highest expression level in okra leaf, followed by root and stem, which reflected its tissuespecific expression pattern. Meanwhile, compared with heat and cold stress, AeMYB1R1 was highly expressed in response to salt stress and drought stress, which suggested that AeMYB1R1 may be the key transcription factor for salt and drought resistance of okra. In summary, this study lays a theoretical basis for the functional study of the AeMYB1R1 gene in okra growth and development and in the mechanism of stress resistance.