Based on the screening of strawberry candidate imprinting gene FaTRG31(turgor responsive protein 31), Using ‘Benihoppe’ (Fragaria×ananassa Duch. ‘Benihoppe’) as the material, we cloned the coding sequence of FaTRG31 gene, and analyzed bioinformatics, tissue expression, promoter and imprinting characteristics to reveal the mechanism of action of this gene and to lay the foundation for the study of expression regulation and biological functions of strawberry inprinted genes. The results showed that: (1) the open reading frame (ORF) of strawberry FaTRG31 gene was 999 bp, encoded 332 amino acids, contains typical NPA structural units and six transmembrane regions, located in the plasma membrane and belonged to AQP(aquaporin) family; Phylogenetic tree analysis showed that FaTRG31 belonged to PIP1(plasma membrane intrinsic proteins subtype 1). (2) Strawberry FaTRG31 was expressed in different tissues, and the expression in endosperm was the highest. The 1 989 bp promoter sequence of FaTRG31 showed that it contained action elements related to endosperm specific expression, a variety of abiotic stress response elements and hormone response elements. (3) The results of strawberry imprinting characterization showed that FaTRG31 had a single peak at the SNP (single nucleotide polymorphism) site in the endosperm was single peak and female parent expression, and the gene was expressed by the female parent in the strawberry endosperm. (4) Analysis after abiotic stress treatments revealed that the strawberry FaTRG31 gene could respond to abiotic stress to varying degrees.