Removal Rate of Benzo[a]pyrene from Soil and Characteristics of Leaf Physiological Response by Ophiopogon japonicus and Carex breviculmis
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    Abstract:

    In order to understand the purification ability of Ophiopogon japonicus and Carex breviculmis on soil benzo[a]pyrene in Beijings garden green space, taking ordinary garden soil as the control, we set up three soil benzo[a]pyrene contamination concentrations of 5.5 mg/kg, 15 mg/kg and 30 mg/kg to study the tolerance, removal effect and characteristics of physiological responses such as photosynthetic pigments and oxidative stress in leaves of two plants on benzo[a]pyrene through pot experiments. The results show that: (1) both plants can survive and have ornamental value at soil benzo[a]pyrene concentrations of 30 mg/kg and below, but the plant height and dry weight of C. breviculmis were significantly inhibited, while only the plant height of O. japonicus was significantly inhibited. (2) Both plants can reduce the concentration of soil benzo[a]pyrene significantly, and the removal rate showed a trend of first increasing and then decreasing with the increase of concentration. The removal rate of O. japonicus was the highest at 5.5 mg/kg (76.9%), and the removal rate of C. breviculmis was the highest at 15 mg/kg (79.6%). (3) With the increase of soil benzo[a]pyrene concentration, the contents of various photosynthetic pigments in the leaves of O. japonicus first decreased and then increased, while C. breviculmis first increased and then decreased, but the changes were significant only under individual treatments. (4) There was no significant difference in MDA content in leaves of O. japonicus under each concentration of benzo[a]pyrene compared with the control group, while C. breviculmis was significantly higher than the control group under each treatment. (5) The contents of hydrogen peroxide and hydroxyl radical in the leaves of O. japonicus and C. breviculmis under each benzo[a]pyrene concentration treatment were basically higher than those in the control group, and among them, the content of hydrogen peroxide under the treatment of 15 mg/kg and 30 mg/kg of O. japonicus and the content of hydroxyl radicals under the treatment of 15 mg/kg concentration increased significantly, and the content of hydrogen peroxide and the content of hydroxyl free radicals under the treatment of 15 mg/kg for C. breviculmis were significantly higher than those in the control group. (6) At each concentration of benzo[a]pyrene in soil, the activities of POD and CAT in leaves of the two plants were not significantly different from those in the control group, and the SOD activity was significantly higher than the control group only under the treatment of 5.5 mg/kg for O. japonicus and under the treatment for 30 mg/kg of C. breviculmis. The study found that both O. japonicus and C. breviculmis can tolerate the soil environment with benzo[a]pyrene pollution concentration lower than 30 mg/kg, and at the same time effectively remove benzo[a]pyrene in the soil; the removal effect of O. japonicus was better than C. breviculmis when the soil benzo[a]pyrene concentration was low, but inferior to C. breviculmis when the soil concentration was high; The strong tolerance of O. japonicus to benzo[a]pyrene soil pollution was closely related to the stable content of photosynthetic pigments in leaves and the lower degree of membrane peroxidation under various pollution concentrations.

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ZHANG Lingqiao, ZHU Mengting, PENG Ying, SONG Linshu, LIU Yan. Removal Rate of Benzo[a]pyrene from Soil and Characteristics of Leaf Physiological Response by Ophiopogon japonicus and Carex breviculmis[J]. Acta Botanica Boreali-Occidentalia Sinica,2022,42(7):1198-1207

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  • Online: August 22,2022
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