This paper describes a new species of Primulina (Gesneriaceae)——P. shaowuensis, from Shaowu County in Fujian Province. Morphological analyses indicated that P. shaowuensis is most similar to P. xiuningensis. The main differences of them are P. shaowuensis flower tube subcampanulate and inflated in the middle; the corolla is milky white; staminodes 3, apex is slightly capitate. The result of phylogenetic analysis used nuclear ribosomal ITS and two plastid markers (atpBrbcL and rpL32trnL) indicated that P. shaowuensis has an independent position on the phylogenetic tree, which supports P. shaowuensis as a new species of Gesneraceae, and P. xiuningensis and P. juliae are its sister group.