Abstract: The study aimed to the phenotypic genetic diversity of 146 kiwifruit germplasm resources in Guizhou Province, and provide reference for breeding and utilization of kiwifruit resources in Guizhou Province. In this study, 48 phenotypic traits of 146 kiwifruit germplasm resources from different regions were described and assigned and 9 quantitative traits such as fruit weight were combined with principal component analysis and cluster analysis to comprehensively evaluate the phenotypic diversity of kiwifruit germplasm resources in Guizhou. The results showed that there was abundant diversity in 57 qualitative and quantitative traits were rich in diversity and variability; The Shannon"s genetic diversity index of 146 resources is 1.28, It can be seen from the genetic diversity index of each region that the evolution process is from the region with high genetic diversity index to the region with low genetic diversity index;. The results of principal component analysis showed that the accumulative contribution rate of the first 14 principal components was 75.076%. The accumulative contribution rate of the first 6 principal components was 51.911%. Improve the efficiency of variety selection and evaluation, the descriptive indexes of kiwifruit germplasm resources can be simplified into 18 characters, including hair on annual branch, fruit hair; Cluster analysis showed that the Euclidean distance of kiwifruit germplasm in Guizhou was between 1.00-25.00. And 146 kiwifruit resources were divided into 6 groups at D=19.25. This paper effectively revealed the rich diversity and genetic relationship of wild kiwifruit germplasm resources in Guizhou Province, and defines the distinguishing characteristics of characters among various groups of kiwifruit and the geographical distribution of some excellent resource characters, which is great significance for the identification, protection, utilization and genetic improvement of kiwifruit germplasm resources in Guizhou.