To explore the calcium contents, components and distribution characteristics in organs of Vitis quinquangularis Rehd. in Karst area, and to reveal its calcium requirement characteristics and its adaptation mechanism to high calcium soil, we used V. quinquangularis in Karst area of Guizhou as the material in the experiment. The pH valve and exchangeable Ca content in soil, Ca, Mg and calcium component contents in root, stem and leaf of V. quinquangularis in 40 samples were tested. The correlation between exchangeable Ca content in soil and different calcium component contents were analyzed. The calcium crystals on the leaf surface and in root, stem and leaf of V. quinquangularis were observed. The results showed that: (1) the distribution of calcium content in organs of V. quinquangularis was leaf > root > stem, and its distribution characteristic is similar to that of calcicole. (2) The contents of main calcium components in root, stem and leaf of V. quinquangularis from high to low were HClCa, NaClCa, H₂OCa, HACCa, ALCCa, ResCa, but slightly different in stem. The proportion of HClCa in all calcium components and calcium content was the highest in root, stem and leaf, followed by NaClCa. (3) The content of Ca+Mg in leaf of V. quinquangularis ranged from 1.30% to 4.07%, and the majority content ranged from 3.0% to 4.0%, which showed that the characteristic of high Ca+Mg content in leaf of calcicole. (4) There were significant or extremely significant correlations between the contents of various calcium components in V. quinquangularis in Karst area and the exchangeable Ca content in soil. (5) A large number of calcium oxalate crystals were found in leaf and root of V. quinquangularis by scanning electronic microscope, which can be excreted through the stomata in leaf. The studies showed that V. quinquangularis in Karst area was calcicole, which had strong adaptability to high calcium environment. A large number of calcium enriched in leaf, and a large amount of HClCa and NaClCa stored in plants. It played an important role in adapting to high calcium environment that calcium storage characteristic and stomatal calcium excretion behavior.