bHLH93 transcription factor is involved in regulating the plant growth and development and various physiological processes in plants. The VvbHLH93 gene was cloned from Cabernet Sauvignon by RTPCR and analyzed by bioinformatics. The expression of VvbHLH93 in different tissues and fruits at different developmental stages was analyzed by qRTPCR, which laid a foundation for further exploring the function and mechanism of VvbHLH93 gene. The results showed that: (1) VvbHLH93 was cloned successfully. The full length cDNA of VvbHLH93 was 1 319 bp, containing a 939 bp open reading frame which encoded 312 amino acids. The relative molecular weight of VvbHLH93 was 35.18 kDa, and its isoelectric point was 4.68. There was no signal peptide or transmembrane domain in this protein, belonging to the bHLH family. (2) bHLH93 in Vitis vinifera L. was closely related to that in Nelumbo nucifera. VvbHLH93 possesses an acidic region in C terminus rich in serine and threonine putative phosphorylation sites. The promoter of VvbHLH93 gene contained light response element, low temperature, drought and GA response element. (3) qRTqPCR analysis showed that the expression of VvbHLH93 in Cabernet Sauvignon was tissuespecific, it was not expressed in leaves, and had the highest expression in stems. The expression level of VvbHLH93 was decreased continuously with the development and maturity of fruit, and it was basically 0 in the 5th week after young fruit stage (> 2 mm in diameter), showing a unimodal downward trend during seed development. Compared with the control group, the expression of VvbHLH93 was significantly decreased under low temperature stress, salt stress, high temperature stress and full irrigation stress. The study speculated that VvbHLH93 may be a negative transcription factor of grape stress resistance.