In order to study the capability and mechanism of different grape rootstocks under salt stress, we used 13 grape rootstocks as experimental materials. The grapevines were supplied with 1/2 Hoaglands nutrient solution with 100 mmol/L NaCl, grapevine rootstocks which were not treated with 100 mmol/L NaCl were used as control. After 20 fully expanded true leaf, we investigated the relative conductivity, osmotically regulates substance content, MDA content, antioxidant enzyme activities of leaves and roots. The results showed: (1) the leaves of the ‘18808’, ‘3309C’ and ‘140R’ treatment groups were almost completely shed, and a small number of leaves fell off in the ‘Beta’, ‘5BB’ and ‘Dogridge’, and the leaf wilting phenomena of ‘10114’ and ‘110R’ were the least until grape rootstock leaves to the end of the salt stress treatment. (2) Under salt stress, the relative conductivity of leaves of the varieties with poor salt tolerance such as ‘3309C’ and ‘140R’ was greater than 90%, and the relative conductivity of leaves of the varieties with strong salt tolerance such as ‘10114’ and ‘110R’ was about 60%. At the same time, with the increase in salt stress treatment days, the MDA content of ‘110R’ showed a clear upward trend. (3) Under salt stress, the higher salttolerant varieties of ‘10114’ and ‘110R’ produced more of osmoregulatory substances such as soluble proteins, soluble sugars and proline. The ‘3309C’ cultivar with poor salt tolerance has accumulated more reactive oxygen species, causing significant lipid film peroxidation, which seriously affects the normal metabolism of rootstock. (4) Under salt stress, ‘10114’ improved the activities of antioxidant enzymes such as SOD and POD, removed excessive reactive oxygen in the body and maintaining the stability of the cell membrane. The results show that grape rootstock varieties ‘10114’ and ‘110R’ can actively accumulate more osmotic regulatory substances under salt stress, enhance their antioxidant enzyme activities, show strong osmotic regulation and antioxidant capacity, and effectively resist the damage caused by salt stress. Therefore, it can be used as a rootstock variety in viticulture in the salinized areas of the northwest region.