Based on the coldresistant transcriptome data of ‘Dingjiaba Liguangtao’, we cloned peach 4coumarateCoA ligase gene (Pp4CL2) by RTPCR and analyzed by bioinformatics and transformation model plants Arabidopsis thaliana and tobacco, so as to analyze the cold resistance mechanism of‘Dingjiaba Liguangtao’. The results showed that: (1) Pp4CL2 gene (accession number: LOC18792923) of peach was successfully cloned, and its cDNA sequence was 1 635 bp, encoding 544 amino acid residues, and has the conserved domain of 4CL gene family. (2) The secondary structure analysis showed that Pp4CL2 protein consists of four secondary structures, of which alpha helix accounts for 30.51%, beta turn accounts for 7.35%, random coil and extended strand account for 41.54% and 20.59% respectively. (3) Analysis of cisacting elements showed that the upstream promoter region of Pp4CL2 gene contained light, low temperature and many hormone response elements. (4) Phylogenetic analysis of Pp4CL2 sequence showed that Pp4CL2 of peach has the highest protein similarity with apricot (Prunus armeniaca), sweet cherry (Prunus avium) and plum (Prunus mume), which are 99.08%, 97.98% and 96.14% respectively. (5) The transformation vector Pp4CL2PRI101 was successfully constructed and transgenic A. thaliana and tobacco plants were identified by PCR. (6) Compared with wild type, transgenic A. thaliana and tobacco had higher relative expression level Pp4CL2 gene, lighter degree of cold injury, higher osmoregulation substance content and antioxidant enzyme activity, and stronger tolerance to low temperature. The study has shown that overexpression of Pp4CL2 gene can enhance the tolerance of plants to low temperature, and it is speculated that Pp4CL2 gene plays an important role in the response of ‘Dingjiaba Liguangtao’ to low temperature stress.