In order to explore the effect of H+- pyrophosphatase coding gene on phosphorus absorption and resistance of sugar beet, and to realize the utilization of excellent genes in sugar beet genetic engineering. In this study, AVP1 gene encoding vacuolar membrane H+- pyrophosphatase of Arabidopsis thaliana was used to transform sugar beet, and the low phosphorus tolerance, salt tolerance and drought resistance of transgenic sugar beet were analyzed. The results showed that AVP1 gene could be expressed in the roots and leaves of sugar beet plants, and the expression level increased under stress; Under the condition of controlling phosphorus supply, the phosphorus content of transgenic sugar beet was significantly higher than that of wild type; Under drought and salt stress, AVP1 gene increased significantly in transgenic sugar beet. Under 200 mmol · L-1 NaCl treatment or 7 days after natural drought, the growth of transgenic plants was inhibited, but the degree of inhibition was significantly lower than that of wild type plants. In addition, with the aggravation of salt and drought stress, compared with wild type plants, the MDA content of transgenic plants is lower and the proline content is significantly increased. AVP1 gene can reduce the damage of stress to the cell membrane of sugar beet, improve the osmotic adjustment ability of sugar beet cells, thereby enhancing the salt tolerance and drought resistance of sugar beet.