The Actinidia chinensis Aux/IAA gene family was identified and analyzed by bioinformatics in this study. Their physicochemical properties, structural characteristics, synteny relationships were analyzed by bioinformatics, tissue-specific expression patterns and exogenous hormones stress responses were also analyzed by qRT-PCR, which lay the foundation for elucidation the biological function of Aux/IAA family during kiwifruit development. The results showed that: (1) 50 Aux/IAA candidate genes were identified in the A. chinensis genome and they were located on twenty-one chromosomes, and with Chr 23 residing the largest number of 11 Aux/IAA genes (AcIAAs). The amino acid size, molecular weight, and isoelectric point ranged from 125-391 aa, 14.06-42.48 kD, 4.33-9.51, and 0-5, respectively. Phylogenetic analysis divided these genes into nine subfamilies. (2) Most AcIAAs contained 4 different conservative domains, most of the members contain Ⅱ, Ⅲ and Ⅳ domains, and some genes lack Ⅰ domain. There were 1-5 introns in the AcIAA genes based on the gene structure analysis. Also, 23 pairs of AcIAA genes were found underwent segment duplications, which including 20 pairs of fragment repeats and 3 pairs of tandem repeats. Moreover, thirty-six pairs orthologous gene were observed between A. chinensis and Arabidopsis. (3) Stress responses, hormone response and tissure specific related cis-elements were abundant in the promoter region of the AcIAA genes. Subcellular localization analysis indicated that most AcIAA located in the cell nuclear. (4) qPCR analysis showed AcIAAs showed tissue-specific expression，and differential response time and intensity under exogenous hormones treatment. Most of the genes were down-regulated at the early stage of treatment, while the gene AcIAA1a and AcIAA18a were up-regulated. The difference of response mode showed that the Aux/IAA family genes may had different functions during the regulation of A. chinensis development. The research suggests that Aux/IAA family genes have functional diversity in A. chinensis, and some of the genes with gene duplication show similarity in tissue expression patterns. It is speculated that there may be redundancy in function of Aux/IAA genes, or gene sequence deletion or gene duplication due to environmental stress in the evolutionary process of A. chinensis.