Autophagy-related gene ATG8 play an important role in regulating plant growth and development and stress responses. This study will analyze the distribution, structure and evolution of eggplant ATG8 using bioinformatics and explore the expression patterns in different tissues and under exogenous hormones and cold stress. A total of seven ATG8 genes are identified in eggplant , which are distributed on six chromosomes. Physicochemical analysis show that the proteins encoded by ATG8 genes contain 118~166 amino acid residues and the isoelectric point varies from 6.29 to 9.16. The analysis of gene structure and conserved motif suggest that the members of ATG8 gene subfamily have conserved gene structure and protein motif. The promoter regions contain a variety of cis-acting elements for multiple hormones and stresses responses. Three pairs of ATG8 genes have synteny relationship in eggplant. There are ten and eleven synteny relationships between eggplant and Arabidopsis and tomato, respectively. Gene expression profiling in tissues show that ATG8 mainly express in different parts of flower, indicating that ATG8 may be related to the development of eggplant flower. Moreover, the results of expression pattern analysis reveal that seven ATG8 genes respond to cold stress and exogenous hormones including ABA, MeJA and SA to some extent, which indicated that ATG8 gene subfamily play a vital role in eggplant growth and development, stress and hormone response.