OsGRF6 belongs to the GRF family and is involved in the regulation of plant growth and development and tolerance to abiotic stress. In order to study the molecular mechanism of OsGRF6 to regulate the primary root development of rice, the transcription factor gene OsGRF6 was cloned from the rice variety ''ZH11''. The evolutionary tree construction and promoter analysis were carried out. The genotype analysis, qRT-PCR identification and rice phenotype observation of OsGRF6-OE and osgrf6 were carried out. The binding sequence of OsGRF6 was detected through yeast one-hybrid assays, and the candidate target genes downstream of OsGRF6 were further analyzed through ChIP-seqs and RNA-seqs data. The results show that: (1) OsGRF6 contains a QLQ and a WRC conservative functional domain, which is closely related to AtGRF1 and AtGRF2 and OsGRF7. The OsGRF6 promoter region contains multiple abiotic stress and hormone response cis-action elements. (2) OsGRF6 showed a higher level of expression in rice seeds, young spikes and roots than in leaf. Subcellular localization shows that OsGRF6 is located in the nucleus, and OsGRF6 has transcription activation activity. (3) Compared with wild types, overexpression of OsGRF6 transgenic plant materials show a longer primary root length, while loss of function mutant osgrf6 show a shorter phenotype of primary roots. (4) OsGRF6 can bind to CGGCA sequence. (5) Combined with ChIP-seqs and RNA-seqs analysis, it is found that a number of the OsGRF6 target genes were involved in regulating rice root development, such as OsARF7 and OsARF4, etc.