In order to explore the regulation mechanism of suberin biosynthesis in Brachypodium distachyon, we cloned the transcription factor gene BdMYB92 regulating suberin synthesis in the roots of B. distachyon by bioinformatics analysis using Bd21 as the experimental material (GenBank accession number OP497966). The expression pattern of BdMYB92 gene in different tissues of B. distachyon and its response to six abiotic stress treatments (drought in the air, simulated drought in the 20% PEG-6000, 4 ℃ cold treatment, 200 mmol/L NaCl, 100 μmol/L ABA and mechanical damage) were analyzed by quantitative PCR. The interaction between BdMYB92 protein and the promoter of BdFAR4 gene was verified by dual-luciferase report assays and yeast one-hybrid. The results showed: (1) the full-length cDNA of BdMYB92 was 1 343 bp, with an open reading frame was 990 bp, encoding 329 amino acids. The molecular weight of the protein was 36.4 kD, and the theoretical isoelectric point was 5.54. (2) The subcellular localization results confirmed that BdMYB92 was located in the nucleus. (3) Quantitative PCR analysis showed that the expression of BdMYB92 was significantly higher in the root than in other tissues such as sheats, nodes, spikelets and internodes. All six abiotic stress treatments could induce the up-regulated expression of BdMYB92, and the response to drought stress was the fastest, indicating that BdMYB92 was involved in the response to stresses in B. distachyon. (4) The results of dual-luciferase report assays and yeast one-hybrid showed that BdMYB92 interacted with the promoter region of the suberin synthesis gene BdFAR4 and could directly regulate the transcription expression of the suberin synthesis gene BdFAR4. It is speculated that BdMYB92 may interact with other suberin synthesis genes in B. distachyon, thereby regulating the deposition of suberin in roots.