MYB transcription factors play a major regulatory role in plant anthocyanin biosynthesis. In order to explore the role of MYB transcription factor in the formation of the blue flower color of Muscari armeniacum，this study used M. armeniacum as the experimental material，based on the local deep mining of the transcriptome database of the research group, and cloned one R2R3-MYB gene named MaMYB114 (GenBank accession number：OQ615377). For bioinformatics analysis and functional validation of heterologous transformed tobacco. The results showed that：(1) The open reading frame of MaMYB114 has a total length of 714b p，encoding a protein of 425 amino acids；Sequence alignment showed MaMYB114 protein contains the R2R3 conserved domain，indicating that it belongs to the AN2 subfamily of the R2R3-MYB family. (2) The results of subcellular localization and transcriptional self-activation confirmed that MaMYB114 was located in the nucleus and had transcriptional autoactivation activity，which was consistent with the general characteristics of transcription factors. (3) Real-time quantitative PCR analysis showed that MaMYB114 gene was highly expressed mainly in flowers，and the expression pattern was tissue-specific. Combined with the change patterns of anthocyanin content at different times，it was found that MaMYB114 was highly expressed in flower buds with high anthocyanin content during the coloring stage，indicating that MaMYB114 participated in the synthesis process of anthocyanins in grape hyacinths. (4) Overexpression of MaMYB114 promotes anthocyanins accumulation in tobacco, indicating that MaMYB114 positively regulates anthocyanin synthesis. This study indicates that the MaMYB114 gene may be an important regulatory gene involved in the formation of the blue color of grape hyacinth.